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We can see that the typical overlap of each and every gene isn’t homogeneous; Phy C gene had 341.83 overlaps, ACC1 gene 793.03, and Q gene 1764.03.
That is certainly because the PCR samples concentration we mixed was not under the identical uniformity.
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However the assembled reads have a lot more proportion of zero-cut; more than 65 reads were zero-cut.
Obviously the nonassembled reads have the longest length cut than the other 3 reads, which illustrated that the reads that Pub Med ID: cannot be assembled from original reads have been of reduced top quality than the reads which will be assembled.
In future investigation, the new MAF threshold must be calculated primarily based around the new sequence result.
As designed, the assembled reads have high quality and once they are aligned to reference genes, they may execute a lot more quality than others reads.
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